Effects of composite microbial inoculant on aerobic composting of spectinomycin mycelial residue

DAI Yanqun; LI Xiufen; XU Zuya; HUA Zhaozhe

doi:10.16085/j.issn.1000-6613.2026-1390

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Effects of composite microbial inoculant on aerobic composting of spectinomycin mycelial residue

更新时间：2026-10-16

- Effects of composite microbial inoculant on aerobic composting of spectinomycin mycelial residue
- Chemical Industry and Engineering Progress (2026)
- 作者机构：
  
  1.浙江金华康恩贝生物制药有限公司,浙江金华321016
  2.江南大学环境与生态学院，江苏无锡 214122
  3.江苏省厌氧生物技术重点实验室，江苏无锡 214122
  4.江苏省水处理技术与材料协同创新中心，江苏苏州 215009
- 作者简介：
- 基金信息：
- DOI：10.16085/j.issn.1000-6613.2026-1390
  CLC： X713
- Received：21 August 2026，
  
  Revised：2026-10-12，
  
  Accepted：12 October 2026，
- 稿件说明：
移动端阅览
DAI Yanqun, LI Xiufen, XU Zuya, et al. Effects of composite microbial inoculant on aerobic composting of spectinomycin mycelial residue[J/OL]. Chemical Industry and Engineering Progress, 2026.
DOI：

DAI Yanqun, LI Xiufen, XU Zuya, et al. Effects of composite microbial inoculant on aerobic composting of spectinomycin mycelial residue[J/OL]. Chemical Industry and Engineering Progress, 2026. DOI： 10.16085/j.issn.1000-6613.2026-1390.

摘要

大观霉素生产过程产生大量抗生素菌渣，主要采用烘干再焚烧的方法进行最终处置，高成本及高碳排始终是制约相关抗生素生产企业健康发展的待解难题。该研究以大观霉素菌渣为堆肥主料，探究了由

Enterococcus faecalis

strain和

Bacillus thermolactis

strain构成的“接力型”复合菌剂添加量对堆体理化性质、产物组成、抗生素残留和抗性基因丰度等的影响，解析了大观霉素与抗性基因可能的生物去除机制。菌剂添加量为0.5×10

CFU/g时，堆体55.0°C以上高温持续时间更长，产物组成与性质优于《有机肥料》（NY/T 525–2021）；残留大观霉素未检出，抗性基因去除率较空白对照提高了38.5%；门水平上，高温结束堆体中

Bacillota

明显富集，

Uroviricota

则受到抑制，有利于堆体无害化；对大观霉素失活去除贡献度较高的属水平物种为

Mycolicibacterium

和

Alkalilimnicola

；腐熟结束时，0.5×10

CFU/g堆体中各类碳水化合物活性酶的丰度上调了1.2~1.8倍，菌剂添加有利于抗生素、抗性基因与有机质的共代谢去除。该研究为氨基糖苷类抗生素发酵菌渣肥料化提供了微生物学依据，也为其资源化利用提供了技术基础。

Abstract

During the production of spectinomycin

a large amount of antibiotic fermentation residue is generated. The primary disposal method involves drying followed by incineration

but the high cost and carbon emissions associated with this process remain persistent challenges that hinder the healthy development of related antibiotic-producing enterprises. In this study

spectinomycin fermentation residue was used as the main substrate for aerobic composting. The effects of the dosage of a composite microbial agent

composed of

Enterococcus faecalis

strain and

Bacillus thermolactis

strain

on the physicochemical properties of the compost pile

product composition

as well as the abundance of antibiotic residues and antibiotic resistance genes (ARGs) were investigated. Additionally

the potential biological removal mechanisms of spectinomycin and ARGs were analyzed. At an inoculum dosage of 0.5×10⁸ CFU/g

the compost pile maintained a high temperature above 55.0°C for a longer duration

and the product compositio

n and properties were superior to the requirements specified in NY/T 525–2021. The residual spectinomycin was not detected

and the removal rate of ARGs was 38.5% higher than that of the blank control. At the phylum level

Bacillota

was significantly enriched in the pile after the high-temperature phase

while

Uroviricota

was suppressed

which was conducive to the detoxification of the compost. At the genus level

Mycolicibacterium

and

Alkalilimnicola

contributed significantly to the removal of spectinomycin. At the end of the maturation phase

the abundance of various carbohydrate-active enzymes in the 0.5×10⁸ CFU/g treatment was upregulated by 1.2–1.8 fold

facilitating the co-metabolic removal of antibiotic

ARGs and organic matter. This study provides a microbiological basis for the fertilizer utilization of aminoglycoside antibiotic fermentation residues and offers a technical foundation for their resourceful reuse.

关键词

Keywords

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